Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Bioworld TT - SDS Running Buffer (Cathode) (10X), 1 L
Product DescriptionTris-Tricine (TT)-SDS running buffer has a 10X concentration and a pH of 8.3. It contains 1M Tris-base, 1M Tricine and 1% SDS.The tricine system requires SDS in sample and running buffers for best results. Tricine-SDS PAGE is commonly used to separate proteins that range in molecular weight between 1 – 100 kDa but is preferred for the resolution of proteins smaller than 30 kDa.
Bioworld TAE Buffer (50X), 4 L
Product DescriptionTAE (Tris-Acetate-EDTA) buffer liquid concentrate has a 50X concentration and is filtered through a 0.22 µm membrane. It has a pH of 8.5 ± 0.1. A concentration of 1X contains 0.04M Tris, 0.02M acetate, and 0.001M EDTA. TAE is used for electrophoresis of nucleic acids in agarose and polyacrylamide gels. It can be used for both genomic and large supercoiled DNA, and can also be used as both a running buffer and a gel preparation buffer. It is recommended for resolution of RNA and DNA fragments larger than 1500 bp, for genomic DNA and for large supercoiled DNA. TAE buffer has a relatively low buffering capacity and therefore replacement of the buffer during prolonged electrophoresis is recommended.
Bioworld BES (2-(Bis(2-hydroxyethyl) amino) ethanesulfonic acid) Buffer, 0.5M, pH 6.0, 250 mL
Synonym: N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium salt, BES sodium saltBelongs to the class of Good buffers, as described in the research of Dr. Norman Good et al. (1966), optimized for molecular biology and biochemistry applications.The characteristics of a Good′s buffer include: pKa between 6.0 and 8.0, high solubility, non toxic, limited effect on biochemical reactions, very low absorbance between 240-700 nm, enzymatic and hydrolytic stability, minimal changes due to temperature and concentration, limited effects due to ionic or salt composition of the solution, limited interaction with mineral cations and limited permeability of biological membranes.Technical Information:pKa (25 °C): 7.1 (Free acid)Warning: For laboratory use only.Not fit for agricultural, household, clinical use or human consumption.Appearance: LiquidPH Value: 6.0
Alkali Scientific TRIS CAPS Buffer 10X Solution, 2 x 500 Milliliter Bottles
TRIS CAPS Buffer 10X Solution is designed for use in capillary electrophoresis and other advanced electrophoretic techniques. This formulation, available in two 500mL bottles, ensures convenient preparation and consistent results during capillary separation of charged biomolecules. The 10X solution can be diluted to create the working concentration needed for various electrophoresis applications. TRIS CAPS buffer is particularly useful in separating proteins and nucleic acids in a high-resolution format. This buffer is often used in protein and DNA/RNA electrophoresis protocols, providing stable pH conditions that enhance separation and improve sensitivity during detection.
Bioworld ACE TBE w/ 7M Urea, pH 8.6, 100 mL
Product DescriptionA.C.E. TM TBE (Tris-Borate-EDTA) buffer comes with 7M of Urea with a pH level of 8.6. A.C.E. TM buffers deliver consistent and reproducible results because they are manufactured with the highest quality reagents in nuclease free water. Product is shipped at room temperature.
![Bioworld ACES (N-[carbamoylmethyl]-2-Aminoethane-sulfonic acid) Buffer, 0.5M, pH 6.2, 500 mL](https://assets.fishersci.com/TFS-Assets/CCG/product-images/50255312.jpg-250.jpg)
Bioworld ACES (N-[carbamoylmethyl]-2-Aminoethane-sulfonic acid) Buffer, 0.5M, pH 6.2, 500 mL
Synonym:N-(2-Acetamido)-2-aminoethanesulfonic acid; N-(Carbamoylmethyl)-2-aminoethanesulfonic acid; N-(Carbamoylmethyl)taurineACES is one ofGood's buffersdeveloped in the 1960s to provide buffers withpHranging from 6.15-8.35 for use in various applications. Azwitterionicbuffer with apKaof 6.9, it is often used as abuffering agentinbiologicalandbiochemicalresearch. ACES had been used to develop buffers for bothagaroseandpolyacrylamide gel electrophoresis.A saturated solution contains 0.22 M at 0°C. ACES binds Cu2+, but not significantly Mg2+, Ca2+or Mn2+.Appearance: LiquidHS Code : 29241900Melting Point: >220 °C (dec.)(lit.)PH Value: 6.0
![Bioworld ACES (N-[carbamoylmethyl]-2-Aminoethane-sulfonic acid) Buffer, 0.5M, pH 7.0, 100 mL](https://assets.fishersci.com/TFS-Assets/CCG/product-images/50255316.jpg-250.jpg)
Bioworld ACES (N-[carbamoylmethyl]-2-Aminoethane-sulfonic acid) Buffer, 0.5M, pH 7.0, 100 mL
Synonym:N-(2-Acetamido)-2-aminoethanesulfonic acid; N-(Carbamoylmethyl)-2-aminoethanesulfonic acid; N-(Carbamoylmethyl)taurine ACES is one ofGood's buffersdeveloped in the 1960s to provide buffers withpHranging from 6.15-8.35 for use in various applications. Azwitterionicbuffer with apKaof 6.9, it is often used as abuffering agent inbiologicalandbiochemicalresearch. ACES had been used to develop buffers for bothagaroseandpolyacrylamide gel electrophoresis.A saturated solution contains 0.22 M at 0°C. ACES binds Cu2+, but not significantly Mg2+, Ca2+or Mn2+. Appearance: LiquidHS Code : 29241900Melting Point: >220 °C (dec.)(lit.)PH Value: 7.0
Medchemexpress LLC Hepes, NaCl, sucrose, pH 7.2 buffer | 50 ML
This physiological buffer is composed of 20 mM HEPES, 150 mM NaCl, and 240 mM Sucrose, adjusted to pH 7.2. It is used to resuspend human erythrocyte ghost preparations. The buffer serves as the final resuspension medium for purified human right-side-out and inside-out erythrocyte ghosts.
- Used as a biochemical assay reagent
- Aids in the resuspension of human erythrocyte ghost preparations
- Appearance is liquid
- Color is colorless to light yellow
- Storage in pure form: -20°C for 3 years, 4°C for 2 years
- Storage in solvent: -80°C for 6 months, -20°C for 1 month
Crescent Chemical Co Inc TRIS BUFFER PH 7.5 1 M SOLN
Tris buffer pH 7.5, 1 M solution molecular biology grade DNase, RNase, Protease not detected. The pH value of Tris buffer is temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03 - 0.05 unit
Teknova 5X TBE DNA Sequencing Grade Solution. 4L, Non-Sterile. 4 pack.
445mM Tris, 445mM Boric Acid, 10mM Ethylenediaminetetraacetic acid (EDTA)
Alkali Scientific Tris Acetate Ultra Pure, 5 Kilograms
Tris Acetate Ultra Pure is a highly pure buffering agent used for creating stable pH conditions in laboratory applications. This 5-kilogram package of tris(acetate) is particularly useful in electrophoresis applications such as DNA and RNA gel electrophoresis. Its ultra-pure grade ensures that it will not interfere with sensitive experiments, making it ideal for molecular biology procedures requiring precise pH control. The tris acetate buffer is widely used in buffer systems for gel electrophoresis, enzymatic reactions, and other applications where stable buffering is critical for optimal results. Its high purity makes it ideal for research applications that demand reliability and accuracy.
![Alkali Scientific PIPES [Piperazine N N' bis(2 ethanesulfonic acid)], 500 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659953.jpg-250.jpg)
Alkali Scientific PIPES [Piperazine N N' bis(2 ethanesulfonic acid)], 500 G
PIPES (Piperazine N,N' bis(2 ethanesulfonic acid)) is a biochemical reagent commonly used to prepare buffer solutions that maintain a stable pH environment in various laboratory experiments. This 500g formulation is perfect for high-volume applications in research involving protein purification, enzyme assays, and microbiological cultures. PIPES provides reliable pH stability around 7.0 and is widely used in studies involving proteins, cells, and biological macromolecules. Its high buffering capacity makes it ideal for maintaining consistent experimental conditions during cell growth, enzymatic reactions, and protein analysis, ensuring accurate and reproducible results.
Alkali Scientific Saline 0.45% 2mL, 10/Pk
Saline 0.45% is a sterile sodium chloride solution commonly referred to as half normal saline. With a salt concentration lower than physiological saline, it is considered hypotonic relative to most biological cells. This property allows water to move into cells through osmosis, causing them to swell under controlled laboratory conditions. Such behavior is useful in certain cytological and cell preparation procedures where swelling assists with cell lysis or chromosome spreading for microscopic examination. The solution is also used for rehydrating freeze dried reagents, enzymes, or antibiotics when lower ionic strength is required. In laboratory workflows it may also serve as a gentle washing or dilution solution in molecular biology and biochemical procedures.
![Alkali Scientific PBS [Phosphate Buffered Saline], 1X Solution, 4.5mL Pre Filled Tubes, Sterile, 36 Tubes per Case, 10mL Tube](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659891.jpg-250.jpg)
Alkali Scientific PBS [Phosphate Buffered Saline], 1X Solution, 4.5mL Pre Filled Tubes, Sterile, 36 Tubes per Case, 10mL Tube
PBS (Phosphate Buffered Saline) 1X Solution is available in pre-filled 4.5mL tubes, designed for use in laboratory assays requiring higher volumes of buffer. This convenient format eliminates the need for measuring and pouring PBS, reducing the risk of contamination and improving efficiency in laboratory workflows. With 36 tubes per case, it is ideal for high-throughput applications such as cell culture, washing, and sample dilution. PBS ensures that the osmotic pressure and pH of biological systems are maintained, providing stable conditions for cells and biomolecules. This product is widely used in molecular biology, immunology, and microbiology experiments.